Aggression assays to determine social structure

Aparna and Doaa are conducing 1000 aggression assays to determine which of the 30 nests we sampled are separate monogyne colonies (so they aggress ants from other nests) and which are part of a polydomous, polygyne, super-colony (so they do not aggress each other).

Each assay is video recorded and then aggressive behaviors are noted and scored. These include biding:

and spraying acid:

Here is a video of an assay between two nests that aggress each other (two monogyne nests). Both ants bite each other and one is spraying acid on the other:

And here is a video of ants from two nests that do not aggress each other (which is pretty boring):

These two nests are part of the supercolony.